Journal: International Journal of Biological Sciences

Article Title: E2F8 regulates the proliferation and invasion through epithelial-mesenchymal transition in cervical cancer

doi: 10.7150/ijbs.37686

Figure Lengend Snippet: E2F8 expression in cervical cancer cell lines and correlation with suppressed cell proliferation. (A, B) The expression levels of E2F8 RNA and protein were significantly higher in some cervical cancer cell lines (CaSki, HeLa, and ME180) compared to that in normal control cells. (C) Using siE2F8, E2F8 knockdown was performed in E2F8-elevated HeLa cell lines. (D) The proliferation of HeLa cells transfected with siE2F8 and negative control siNC was determined using the CCK-8 assay. (E) Using shE2F8, E2F8 knockdown was performed in E2F8-elevated ME180 cell lines. (F) The proliferation of ME180 cells transfected with shE2F8 and negative scrambled control was determined using the CCK-8 assay. Each assay was performed in triplicate. Data are means ± standard deviation. ** P <0.01, ***P<0.001 vs siNC and scrambled control.

Article Snippet: Human epithelioid cervical adenocarcinoma (HeLa) cells were obtained from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Expressing, Control, Knockdown, Transfection, Negative Control, CCK-8 Assay, Standard Deviation

Journal: International Journal of Biological Sciences

Article Title: E2F8 regulates the proliferation and invasion through epithelial-mesenchymal transition in cervical cancer

doi: 10.7150/ijbs.37686

Figure Lengend Snippet: E2F8 promotes cell migration and invasion. (A, C) Wound healing assay observed under the optical microscope was used to determine cell migration using si and shE2F8. E2F8 knockdown was performed in E2F8-high HeLa and ME180 cell lines. (B, D) Cell invasion was observed under the optical microscope. Matrigel invasion assays were used to determine invasion after 48 h in HeLa and ME180 cells. Each assay was performed in triplicate. Data represent means ± standard deviation. ** P <0.01, *** P <0.001 vs. siNC and scrambled control.

Article Snippet: Human epithelioid cervical adenocarcinoma (HeLa) cells were obtained from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Migration, Wound Healing Assay, Microscopy, Knockdown, Standard Deviation, Control

Journal: Molecules

Article Title: Synthesis and Pharmacological Effects of Diosgenin–Betulinic Acid Conjugates

doi: 10.3390/molecules25153546

Figure Lengend Snippet: Cytotoxicity screening tests (IC 50 values (μM), 72 h).

Article Snippet: The compounds 1 – 8 were subjected to the cytotoxicity screening tests on cells of human T-lymphoblastic leukemia (CEM), cells of human breast adenocarcinoma (MCF7), cells of human cervical cancer (HeLa), and human colon carcinoma (HCT 116 cancer cell lines), using normal human fibroblasts (BJ) as reference cell lines.

Techniques:

Journal: ACS Omega

Article Title: Roles of DNA Target in Cancer Cell-Selective Cytotoxicity by Dicopper Complexes with DNA Target/Ligand Conjugates

doi: 10.1021/acsomega.3c03387

Figure Lengend Snippet: Plots of expected enhancement rates in DNA dsb in HeLa cells (A) × (C) shown in Table vs enhancement rates in cytotoxicity against HeLa cells (B) for 1 P1–3 (red line) and 1M 1–3 (purple line). The slopes of the red and blue lines are 0.44 and 0.053, respectively.

Article Snippet: Prof. Kitagishi provided HeLa (human cervical cancer) cells used in this study (Doshisha University, Kyoto, Japan).

Techniques:

Journal: ACS Omega

Article Title: Roles of DNA Target in Cancer Cell-Selective Cytotoxicity by Dicopper Complexes with DNA Target/Ligand Conjugates

doi: 10.1021/acsomega.3c03387

Figure Lengend Snippet: Confocal microscopic images of 1 P1–3 (200 μM) in HeLa cells in the dark on 1 h incubation. Bright-field images (A, E, I). Blue fluorescence indicates the fluorescence of 1 P1 (B), 1 P2 (F), and 1 P3 (J) (λ ex = 405 nm). Red fluorescence is mitochondrial staining by Mito Tracker Deep Red FM (50 nM) (Thermo Fisher) (C, G, K) (λ ex = 640 nm). (D, H, L) Overlay images of panels (A)–(C), (E)–(G), and (I)–(K), respectively. The scale bar is 20 μm.

Article Snippet: Prof. Kitagishi provided HeLa (human cervical cancer) cells used in this study (Doshisha University, Kyoto, Japan).

Techniques: Incubation, Fluorescence, Staining

Journal: ACS Omega

Article Title: Roles of DNA Target in Cancer Cell-Selective Cytotoxicity by Dicopper Complexes with DNA Target/Ligand Conjugates

doi: 10.1021/acsomega.3c03387

Figure Lengend Snippet: In Vitro Cytotoxicity of 1 , 1 X , and Cisplatin against Various Cells in MTT Assay (48 h) (Mean ± SD)

Article Snippet: Prof. Kitagishi provided HeLa (human cervical cancer) cells used in this study (Doshisha University, Kyoto, Japan).

Techniques: In Vitro, MTT Assay

Journal: ACS Omega

Article Title: Roles of DNA Target in Cancer Cell-Selective Cytotoxicity by Dicopper Complexes with DNA Target/Ligand Conjugates

doi: 10.1021/acsomega.3c03387

Figure Lengend Snippet: Data for the Plot of Expected Enhancement Rates ( 1 X / 1 ) in DNA dsb in HeLa Cells (A) × (C) vs Enhancement Rates ( 1 X / 1 ) in Cytotoxicity against HeLa Cells (B) a

Article Snippet: Prof. Kitagishi provided HeLa (human cervical cancer) cells used in this study (Doshisha University, Kyoto, Japan).

Techniques:

Journal: Cancer Cell International

Article Title: Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells

doi: 10.1186/s12935-016-0379-1

Figure Lengend Snippet: Forced expression of TFF3 in cervical cancer cells modulates the expression of malignant progression-related gene markers. a Determination of endogenous expression of TFF3 protein by Western blot in the cervical cancer cells lines CaSki, SiHa, Me180 and Hela and human non-tumor keratinocyte line HaCaT. b Semi-quantitative RT-PCR analysis was used to assess the mRNA levels of TFF3 in SiHa and Hela cells with either forced or depleted expression of TFF3 as described in “ ” section. c Western blot analysis was used to assess the protein levels of TFF3 in SiHa and Hela cells with either forced or depleted expression of TFF3. d , e Quantitative PCR analysis quantifying the change in expression of various genes associated with malignant progression in SiHa-TFF3/Hela cells. Change in gene expression is expressed as fold difference, respectively. Fold change values are representative of three independent experiments

Article Snippet: Human cervical cancer cell lines SiHa, CaSki, Hela, Me180 and human non-tumor keratinocyte line HaCaT were obtained from Nanjing KeyGen Biotech Co, Ltd (Nanjing,China).

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Gene Expression